mre11 rabbit polyclonal antibody pnb 100 142  (Novus Biologicals)

Journal: Oncogene

Article Title: PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53.

doi: 10.1038/onc.2015.63

Figure Lengend Snippet: Figure 4. PML-IV/-V does not affect E1B-55K binding to p53, Mre11 and Daxx. H1299 cells were transfected with 5 μg of pE1B-55K variants and harvested after 48 h before preparing total cell extracts. After immunoprecipitation of endogenous Mre11 and Daxx using Mre11 rabbit pAb pNB 100–142 and rabbit pAb α-Daxx, proteins were resolved by 10% SDS–PAGE and visualized by immunoblotting. Co-precipitated proteins (left panel) and input levels (right panel) of total cell lysates were detected using mAb 2A6 (α-E1B-55K), Mre11 rabbit pAb pNB 100–142, rabbit pAb α-Daxx and mAB AC-15 (α-β-actin). Molecular weights in kDa are indicated on the left, whereas corresponding proteins are labeled on the right.

Article Snippet: Antibodies and protein analysis Primary Abs for HAdV5 proteins included E1B-55K mouse mAb 2A6.139 Primary Abs for detecting of cellular and etopically expressed proteins used, included p53 mouse mAb DO-I (Sigma-Aldrich, Inc., St Louis, MO, USA), PML rabbit pAb NB100–59787 (Novus Biologicals, Inc., Littleton, CO, USA), Mre11 rabbit polyclonal antibody pNB 100–142 (Novus Biologicals, Inc.), PML rabbit pAb NB100–59787 (Novus Biologicals, Inc.), mAb α-Ubc9 (BD Transduction, Heidelberg, Germany), rabbit pAb α-Daxx (Millipore, Darmstadt, Germany), ß-actin mouse mAb AC-15 (Sigma-Aldrich, Inc.) and mouse mAb Flag-M2 (Sigma-Aldrich, Inc.).

Techniques: Binding Assay, Transfection, Immunoprecipitation, SDS Page, Western Blot, Labeling